Biochem/physiol Actions

Fully functional as measured using 5 nM Ipase-T and 1 µM Ub-AMC (Cat. No. 662075)

General description

Native, rabbit isopeptidase T. A member of the higher molecular weight group of ubiquitin C-terminal hydroxylases (UCHs) involved in the hydrolysis of isopeptide linkages of polyUb chains. Ipase-T activity is regulated by ubiquitin levels. Plays a major role in ubiquitin recycling and in the regulation of 26S proteasome activity.

Native, rabbit isopeptidase T. A member of the higher molecular weight group of ubiquitin C-terminal hydrolases (UCHs) involved in the hydrolysis of isopeptide linkages of polyUb chains. Ipase-T activity is regulated by ubiquitin levels. Plays a major role in ubiquitin recycling and in the regulation of 26S proteasome activity. Recommended reaction buffer: 25 mM HEPES, 10 mM DTT, 25°C, pH 7.5. Pre-incubate the enzyme for 15 minutes with 10 mM DTT to achieve maximum activity.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Dang, L.C., et al. 1998. Biochemistry 37, 1868.Melandri, F.D., et al. 1996. Biochemistry 35, 12893.Stein, R.L., et al. 1995. Biochemistry 34, 12616.Pickart, C.M., and Rose, I.A. 1986. J. Biol. Chem. 261, 10210.

Packaging

25 µg in Plastic ampoule

Physical form

In 150 mM NaCl, 50 mM HEPES, 1 mM DTT, pH 7.6.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Warning

Toxicity: Standard Handling (A)